Journal: Cell reports
Article Title: LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation.
doi: 10.1016/j.celrep.2023.113479
Figure Lengend Snippet: Figure 6. LINC00921 activates Wnt/b-catenin signaling through MED23 in NSCLC cells (A) WikiPathways analyses for genes significantly downregulated in PC9 cells (sh921-1 and sh921-2 vs. NC). (B) The relative mRNA expression levels of c-Myc, CCND1, BMP4, and b-catenin in NSCLC cells with stably enforced LINC00921 expression or LINC00921 depletion.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies MED13L Bethyl Cat# A302-421A; RRID:AB_1907304 H3K27ac Abcam Cat# ab4729; RRID:AB_2118291 P300 Santa Cruz Cat# sc-48343X Ki-67 Abcam Cat# ab15580; RRID:AB_443209 NUDT21 Proteintech Cat# 66335-1-Ig; RRID:AB_2881715 METTL16 Proteintech Cat# 19924-1-AP; RRID:AB_10639364 HNRPF Proteintech Cat# 67701-1-Ig; RRID:AB_2882893 EWSR1 Proteintech Cat# 55191-1-AP; RRID:AB_10860907 PRKRA Proteintech Cat# 10771-1-AP; RRID:AB_2170496 EIF2S1 Abcam Cat# ab137626; RRID:AB_2736873 Anti-HA tag Abcam Cat# ab236632; RRID:B_2864361 IgG Invitrogen Cat# 02-6502 TRIP12 Bethyl Cat# A301-814A; RRID:AB_1264344 CPSF4 Proteintech Cat# 15023-1-AP; RRID:AB_2084544 CPSF1 Proteintech Cat# 11031-1-AP; RRID:AB_2245276 CSTF1 Proteintech Cat# 10064-2-AP; RRID:AB_2261145 PCF11 Proteintech Cat# 23540-1-AP; RRID:AB_2879293 MED23 Bethyl Cat# A300-425A; RRID:AB_2142309 TNFRSF6B Proteintech Cat# 24999-1-AP; RRID:AB_2879838 c-MYC Abcam Cat# ab32072; RRID:AB_731658 BMP4 Abcam Cat# ab235114 b-CATENIN Abcam Cat# ab227499 CCND1 Proteintech Cat# 60186-1-Ig; RRID:AB_10793718 ACTIN Proteintech Cat# 20536-1-AP; RRID:AB_10700003 CoraLite488-conjugated Affinipure Goat Anti-Mouse IgG(H + L) Proteintech Cat# SA00013-1; RRID:AB_2810983 CoraLite594-conjugated Goat Anti-Rabbit IgG(H + L) Proteintech Cat# SA00013-4; RRID:AB_2810984 Bacterial and virus strains DH5a competent cell Cwbiotech Cat# CW0808S Biological samples Human lung cancer tissues Shandong Cancer Hospital and Institute N/A Human lung cancer tissues Huaian No. 2 Hospital (Nan Jing) N/A Chemicals, peptides, and recombinant proteins RPMI 1640 medium Gibco Cat# C11875500BT Fetal Bovine Serum Gibco Cat# 1347575 DMEM medium Gibco Cat# C11995500BT INTERFERin Polyplus Transfection Cat# 101000028 jetPRIME Polyplus Transfection Cat# 114-07 Evo M-MLV RT Kit Accurate Biology Cat# AG11706 (Continued on next page) Cell Reports 42, 113479, December 26, 2023 17
Techniques: Expressing, Stable Transfection